Pituitary Gland


Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources.

Resources on M+Box

AtlasWheater’s, Pituitary Gland
TextRoss and Pawlina, Overview of Endocrine Glands Ross and Pawlina, Pituitary Gland and Hypothalamus


  1. Be able to identify the parts of the pituitary gland, and describe their embryological origin.
  2. Name the cell types that produce the various hormones of the anterior pituitary, and know whether they are acidophils or basophils.
  3. Describe the role of hypothalamic neurosecretion in the function of the posterior pituitary, and in the regulation of the anterior pituitary, and recognize the histological structures involved in these functions.


These slides show mostly pars distalis, but also have a small area of neurohypophysis (either pars nervosa or infundibular stalk) on one side (see slide orientation diagrams) and are stained in alternate sets with H&E or with Masson trichrome. The two classes of anterior pituitary cells (acidophils and basophils) are most easily distinguished with Masson trichrome staining, but you should also see how they look with routine H&E staining.

A. Pars distalis (aka anterior pituitary or adenohypophysis)

Slide 226 Pituitary gland Masson cross Webscope Imagescope orientation
Slide 226 Pituitary gland H&E cross Webscope Imagescope
Slide 226 Pituitary gland H&E sagittal Webscope Imagescope orientation
Slide 227 Pituitary gland Masson sagittal Webscope Imagescope orientation

Study the pars distalis (anterior lobe) in the slide stained with Masson trichrome #226 pars distalis  Webscope   ImageScope . The cells are arranged in irregular clusters or cords and are distinguishable by their staining as either acidophils, basophils, or chromophobes. The acidophils stain red or orange-red, while the basophils stain various shades of blue or blue-gray. Remember that the acidophils include two different cell types, somatotropes (growth hormone) and mammotropes (prolactin), while the basophils include gonadotropes (FSH and LH), thyrotropes (TSH) and corticotropes (ACTH). ACTH is actually a cleavage product of pro-opiomelanocortin (POMC), which is made by corticotropes and processed primarily into ACTH in these cells. To a lesser extent, corticotropes also produce other signaling factors derived from POMC such as lipotropins (involved in lipid metabolism), endorphins (endogenous opioids that reduce pain perception), and melanocyte stimulating hormone (MSH). Occasional cells in the anterior pituitary show no distinctive staining and are called “chromophobes”. You will only be required to distinguish acidophils from basophils. Your best strategy is first to identify acidophils, which are more distinctively stained, and then the remaining cells are almost entirely basophils. The cells are not uniformly distributed throughout the pars distalis, but instead there are areas where acidophils predominate, other areas where basophils are more numerous, while still other regions may show a more even mixture of acidophils and basophils. INDO264

Note the abundant sinusoidal capillaries (often filled with red blood cells) #226 sinusoidal capillaries  Webscope   ImageScope that lie between the cell cords or clusters. You can appreciate how readily the hormones secreted from the cells can reach the blood. Since collagen stains bright blue with the Masson trichrome method, you can see the delicate connective tissue partitions between cords and around blood vessels.

In the routine H&E-stained sections, you can also identify acidophils and basophils, although the difference is not as obvious as it is with Masson trichrome staining. Here again, you should first identify acidophils, which stain various shades of reddish pink, and then the remaining cells are almost entirely basophils, which vary generally from bluish/grayish-pink to blue. INDO266

B. Pars nervosa (aka posterior pituitary or neurohypophysis)

Slide 227 Pituitary gland monkey Masson cross Webscope Imagescope orientation
Slide 227 Pituitary gland monkey H&E cross Webscope Imagescope orientation
Slide 227 Pituitary gland Masson sagittal Webscope Imagescope

Although the pars nervosa can be found on the human pituitary slides in our collection, the monkey pituitary specimens (H&E and trichrome-stained) contain a significant portion of the pars nervosa (posterior lobe) and are probably better for studying this tissue. The pars nervosa looks like brain tissue, which it is. It is an extension of the brain, composed primarily of nerve fibers (axons) which originate from nerve cell bodies in the hypothalamus and pass to the pars nervosa by way of the hypothalamo-hypophyseal tract and the infundibular stalk. These nerve fibers carry oxytocin and antidiuretic hormone (ADH, vasopressin) to nerve endings, from which they are released into nearby capillaries upon neural stimulation from the hypothalamus. There is not much to see in the posterior lobe in these histological sections. Since there are no neuron cell bodies in this structure, most of the prominent nuclei belong to pituicytes , which are the characteristic glial cells of the pars nervosa. You will also see the nuclei of blood vessel endothelial cells , and fibroblasts which are in the connective tissue around these vessels.

C. Pars intermedia

Slide 227 Pituitary gland monkey Masson cross Webscope Imagescope
Slide 227 Pituitary gland monkey H&E cross Webscope Imagescope
Slide 226 Pituitary gland H&E sagittal Webscope Imagescope
Slide 227 Pituitary gland Masson sagittal Webscope Imagescope

The pars intermedia is very poorly developed in the human pituitary, but is prominent in the pituitaries of most other mammals. For example, in the monkey pituitary, you will see the pars intermedia as a prominent layer several cells thick, lying between the pars distalis and pars nervosa. In some places you may also see a long cleft between the pars distalis and the pars intermedia, which is a substantial remnant of the lumen of the embryonic Rathke’s pouch, an ectodermal outpocketing of the oral cavity which gave rise to both the pars distalis and the pars intermedia. Cells of the pars intermedia also produce POMC (pro-opiomelanocortin), which in these cells is processed primarily into endorphins and MSH (melanocyte stimulating hormone).

In contrast to its substantial presence in other mammals, the pars intermedia of the human pituitary is usually represented merely by a thin layer of basophilic cells that can be seen in both the human trichrome and H&E-stained human sections lying against the pars nervosa, and is probably of little functional importance. Between the pars intermedia and pars distalis are occasional fluid-filled cysts (again visible in both trichrome #227   Webscope   ImageScope and H&E-stained #226   Webscope   ImageScope sections), which are the only vestiges of the lumen of Rathke’s pouch. Although most of the human axial sections in your sets do not show the human pars intermedia very well, the sagittal sections in some of the sets show some indication of it.

D. Infundibular stalk and hypophyseal portal vessels

Slide 229B Infundibular stalk, and hypophyseal portal vessels H&E Webscope Imagescope orientation
Slide 229 Infundibular stalk, hypophyseal portal vessels H&E Webscope Imagescope

This slide shows the hypophyseal portal blood vessels #229   Webscope   ImageScope that carry releasing hormones from the hypothalamus to regulate the release of hormones from the pars distalis of the pituitary. Look for these prominent blood vessels in portions of the pars distalis that extend up around the infundibular stalk. In many of these slides the sinusoidal capillaries #229   Webscope   ImageScope lying between cords of pituitary cells throughout the anterior pituitary are shown particularly well. Scan over the slide, reviewing other features you saw in slides 226 and 227 (e.g. acidophils vs. basophils; neurohypophysis vs. adenohypophysis).


Electron Micrographs